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MOLECULAR ASPECTS OF CELLULAR TRANSFORMATION BY BOVINE PAPILLOMAVIRUS TYPE 1.

The bovine papillomaviruses (BPV) are capable of transforming cells from a wide range of species both in vivo and in vitro. The 69% portion of the BPV-1 viral genome that contains the transforming region, as well as the 31% portion, were cloned in the pBR322 plasmid vector. An extensive restriction endonuclease map of the transforming region was prepared. Using the cloned transforming region as a ³²P probe, BPV-1 coded mRNA transcripts from transformed cells were detected and sized using the northern blot technique. The largest open reading frame of the transforming region of the BPV-1 genome was sequenced using the Maxam and Gilbert chemical method and the amino acid sequence of a protein that this region could code for was presented.

Identiferoai:union.ndltd.org:arizona.edu/oai:arizona.openrepository.com:10150/187069
Date January 1983
CreatorsABRAHAM, JOHN MARTIN.
PublisherThe University of Arizona.
Source SetsUniversity of Arizona
LanguageEnglish
Detected LanguageEnglish
Typetext, Dissertation-Reproduction (electronic)
RightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.

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