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Studies on the regional difference of electrogenic chloride secretion in the cultured rat epididymal epithelium.

by Ka-bik Lai. / Thesis (M.Phil.)--Chinese University of Hong Kong, / Includes bibliographical references (leaves 59-67). / Chapter Chapter I --- Introduction / Chapter I.1 --- Anatomy and functions of the epididym --- p.is / Chapter I.1.1 --- Gross anatomy and functions --- p.1 / Chapter I.1.2 --- Histology of the epithelium and functions of different cell types --- p.3 / Chapter I.2 --- Regional difference / Chapter I.2.1 --- Epithelium --- p.4 / Chapter I.2.2 --- Electrolyte transport --- p.6 / Chapter I.3 --- Short circuit current studies on C1- secretion in the cultured rat epididymal epithelium --- p.7 / Chapter I.4 --- Physiological effects of vasopressin in the epididymis and the male reproductive tract --- p.10 / Chapter I.5 --- Objectives of the study --- p.14 / Chapter Chapter II --- Materials and methods / Chapter II. 1 --- Materials / Chapter II.1.1 --- Chemicals for cell culture --- p.16 / Chapter II. 1.2 --- Chemicals and drugs for short circuit current study --- p.16 / Chapter II. 1.3 --- Materials for preparing pervious supports --- p.16 / Chapter II.2 --- Preparation of solution for short circuit current study --- p.17 / Chapter II.3 --- Preparation of pervious supports for the cultured epithelium --- p.17 / Chapter II.4 --- Culture of rat epididymal epithelial cells / Chapter II.4.1 --- "Dissection of the efferent duct, the initial segment and the cauda epididymidis" --- p.17 / Chapter II.4.2 --- Culture procedures for the epithelium --- p.19 / Chapter II.4.3 --- Confluent epithelial monolayer --- p.22 / Chapter II.5 --- Short circuit current measurement / Chapter II.5.1 --- Mounting of cultured epithelium on Ussing chambers --- p.22 / Chapter II.5.2 --- Experimental setup for zero voltage clamp --- p.25 / Chapter Chapter III --- Results / Chapter III.1 --- Regional differences / Chapter III.1.1 --- Basal bioelectrical properties --- p.29 / Chapter III. 1.2 --- Effects of C1- replacement and C1- channel blocker --- p.29 / Chapter III.1.3 --- Effects of ion transportor inhibitors --- p.33 / Chapter III. 1.4 --- Effect of adenylate cyclase stimulator --- p.33 / Chapter III.1.5 --- Effect of exogenous PGE2 --- p.33 / Chapter III.1.6 --- Effect of Ca2+ ionophore --- p.36 / Chapter III.2 --- Effect of [arg8]-vasopressin / Chapter III.2.1 --- Effect of [arg8] -vasopressin on short circuit current --- p.36 / Chapter III.2.2 --- Effects of C1- replacement and C1- channel blocker --- p.40 / Chapter III.2.3 --- Effect of repeated stimulation with [arg8]- vasopressin --- p.40 / Chapter III.2.4 --- Effects of receptor antagonists --- p.40 / Chapter III.2.5 --- Effects of thapsigargin and trifluoperazine --- p.44 / Chapter III.2.6 --- Effects of indomethacin --- p.44 / Chapter III.2.7 --- Effects of forskolin --- p.47 / Chapter Chapter IV --- Discussion --- p.50 / Chapter Chapter V --- References --- p.59

Identiferoai:union.ndltd.org:cuhk.edu.hk/oai:cuhk-dr:cuhk_319248
Date January 1993
ContributorsLai, Ka-bik., Chinese University of Hong Kong Graduate School. Division of Basic Medical Sciences.
PublisherChinese University of Hong Kong
Source SetsThe Chinese University of Hong Kong
LanguageEnglish
Detected LanguageEnglish
TypeText, bibliography
Formatprint, 67 leaves : ill. (some mounted col.) ; 30 cm.
RightsUse of this resource is governed by the terms and conditions of the Creative Commons “Attribution-NonCommercial-NoDerivatives 4.0 International” License (http://creativecommons.org/licenses/by-nc-nd/4.0/)

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