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Acetyl - CoA karboxylasa - evoluce a inhibice / Acetyl - CoA carboxylase - evolution and inhibition

Abstract
 Acetyl-CoA
carboxylase
(ACC)
is
a
key
enzyme
of
fatty
acid
metabolism
with
multiple
 isozymes
often
expressed
in
different
eukaryotic
cellular
compartments.

 In
agriculture,
inhibitors
of
plastid
ACC
are
used
as
efficient
herbicides
against
grass
 weed.
However,
grass
weed
populations
resistant
to
aryloxyphenoxypropionate
(APP)
and
 cyclohexanedione
(CHD)
herbicides
represent
a
major
problem
for
sustainable
agriculture.
 Using
PCR
and
sequencing
it
was
found
out
that
five
amino
acid
substitutions
in
plastid
ACC
 were
 correlated
 with
 herbicide
 resistance
 of
 Avena
 sterilis
 ssp.
 ludoviciana
 Durieu
 populations
from
the
northern
grain-growing
region
of
Australia:
Trp-1,999-Cys,
Trp-2,027- Cys,
 Ile-2,041-Asn,
 Asp-2,078-Gly
 and
 Gly-2,096-Ala.
 We
 showed,
 using
 a
 yeast
 gene- replacement
 system,
 that
 these
 single-site
 mutations
 also
 confer
 herbicide
 resistance
 to
 wheat
plastid
ACCase:
Asp-2,078-Gly
confers
resistance
to
APPs
and
CHDs,
Trp-2,027-Cys
 and
Ile-2,041-Asn
confer
resistance
to
APPs,
and
Trp-1,999-Cys
confers
resistance
only
to
 fenoxaprop.
These
mutations
are
very
likely
to
confer
resistance
to
any
grass
weed
species
 under
selection
imposed
by
the
extensive
agricultural
use
of
the
herbicides.

...

Identiferoai:union.ndltd.org:nusl.cz/oai:invenio.nusl.cz:330412
Date January 2012
CreatorsChalupská, Dominika
ContributorsČeřovská, Noemi, Plchová, Helena, Vlček, Čestmír
Source SetsCzech ETDs
LanguageCzech
Detected LanguageEnglish
Typeinfo:eu-repo/semantics/doctoralThesis
Rightsinfo:eu-repo/semantics/restrictedAccess

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