Geminiviruses are a group of plant-infecting viruses that include the genera Begomovirus and Curtovirus. Although members of these two genera are similar in regards to their mechanisms of replication and encapsidation, they exhibit differences in movement as well as in transcriptional activation of late gene promoters. This study evaluated protein-protein interactions of the begomovirus AL2 protein and a curtovirus C2 protein using the yeast two-hybrid system. The AL2 proteins of TGMV, BGMV, and CabLCV were evaluated for their ability to self-interact, as well as to interact with heterologous AL2 proteins, and host proteins. The AL2 proteins tested were all shown to contain the ability to self-interact and to interact heterologously with one another. However, interactions between the AL2 proteins and the host proteins could not be assayed due to lack of expression of certain AL2 'bait' proteins. The BCTV C2 protein was also evaluated for self-interaction and interaction with the begomovirus AL2 proteins. Unlike AL2, C2 was not shown to contain the property of self-interaction and the preponderance of the evidence demonstrated that it also did not interact with AL2. However, the BCTV C2 protein was shown for the first time to possess transcriptional activation activity. In addition, self-interaction of TGMV AL2 was tested using a biochemical assay. However, technical problems prevented the detection of TGMV AL2 self-interaction using an immunocapture approach, although such self-interaction was observed using the yeast two-hybrid system.
| Identifer | oai:union.ndltd.org:NCSU/oai:NCSU:etd-08012003-153220 |
| Date | 05 August 2003 |
| Creators | Jernigan, Leigh Lashley |
| Contributors | Ian Petty, Eric Miller, Geraldine Luginbuhl |
| Publisher | NCSU |
| Source Sets | North Carolina State University |
| Language | English |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | http://www.lib.ncsu.edu/theses/available/etd-08012003-153220/ |
| Rights | unrestricted, I hereby certify that, if appropriate, I have obtained and attached hereto a written permission statement from the owner(s) of each third party copyrighted matter to be included in my thesis, dissertation, or project report, allowing distribution as specified below. I certify that the version I submitted is the same as that approved by my advisory committee. I hereby grant to NC State University or its agents the non-exclusive license to archive and make accessible, under the conditions specified below, my thesis, dissertation, or project report in whole or in part in all forms of media, now or hereafter known. I retain all other ownership rights to the copyright of the thesis, dissertation or project report. I also retain the right to use in future works (such as articles or books) all or part of this thesis, dissertation, or project report. |
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