In plants, the generation and supply of methyl units is important in one-carbon (C-1) metabolism, which is essential to all organisms. I have identified a series of cDNA sequences encoding N5, N10-methylenetetrahydrofolate reductase (MTHFR), cobalamin-independent methionine synthase (Met Syn), S-adenosylmethionine synthetase (isoform I, AdoMet Syn2661 and isoform II, AdoMet Syn605), S-adenosylmethionine decarboxylase (SAMDC), serine hydroxymethyltransferase (SHMT) and N5, N10-methenyltetrahydrofolate cyclohydrolase / N5, N10-methylenetetrahydrofolate dehydrogenase (THFC/THFD) in the pathways of generation and supply of methyl units. These are from a cDNA library of mRNA from a susceptible wheat (Triticum monococcum) (Tm) line 441 epidermis, 24 h after inoculation with powdery mildew fungus (Blumeria graminis f. sp. tritici) (Bgt). Phylogenetic tree cluster analysis and subcellular localization prediction by TargetP revealed that MTHFR, Met Syn, AdoMet Syn605, AdoMet Syn2661, SAMDC, and THFC/THFD may be localized in cytosol; SHMT may be localized in mitochondria. Northern blot analysis indicated that expression of MTHFR, Met Syn, AdoMet Syn2661, AdoMet Syn605 and SAMDC genes was up-regulated by powdery mildew infection, abiotic stresses and treatments with stress signal molecules; expression of SHMT and THFC/THFD was either constitutive or down-regulated. These results suggest a close metabolic link between various stresses and the pathways of generation and supply of methyl units in this wheat.
| Identifer | oai:union.ndltd.org:USASK/oai:usask.ca:etd-03162005-182518 |
| Date | 17 March 2005 |
| Creators | Liu, Weiping |
| Contributors | Wei, Yangdou, Selvaraj, Gopalan, Messier, François, King, John, Gray, Gordon R. |
| Publisher | University of Saskatchewan |
| Source Sets | University of Saskatchewan Library |
| Language | English |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | http://library.usask.ca/theses/available/etd-03162005-182518/ |
| Rights | unrestricted, I hereby certify that, if appropriate, I have obtained and attached hereto a written permission statement from the owner(s) of each third party copyrighted matter to be included in my thesis, dissertation, or project report, allowing distribution as specified below. I certify that the version I submitted is the same as that approved by my advisory committee. I hereby grant to University of Saskatchewan or its agents the non-exclusive license to archive and make accessible, under the conditions specified below, my thesis, dissertation, or project report in whole or in part in all forms of media, now or hereafter known. I retain all other ownership rights to the copyright of the thesis, dissertation or project report. I also retain the right to use in future works (such as articles or books) all or part of this thesis, dissertation, or project report. |
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