Anaerobic digestion (AD) is a widespread technology for treating waste streams such as livestock manure. During AD, biogas is produced and subsequently used as renewable energy for certain purposes, such as injection into the natural gas grid or as fuel for transportation. Despite its many advantages, AD can be limited by various factors, including process instability against volatile fatty acids (VFA), nitrogen overloads, or the presence of inhibitors, as well as the need for biogas post-processing to increase its methane content. Therefore, strategies are needed to monitor the AD process, control the effluent quality and upgrade the biogas recovered. Microbial electrochemical technologies (MET) have the potential to optimize AD. MET are systems in which oxidation and/or reduction reactions are catalyzed by electroactive microorganisms (EAM) on the surface of an electrode. Typically, EAM used in AD-MET combinations are dominated by Geobacter spp., that form multilayer biofilms on electrodes (e.g., anodes) used as solid terminal electron acceptors. However, using Geobacter spp. dominated biofilm anodes in AD-MET combinations has so far encountered several hurdles, ranging from biofilm dispersal to inhibition of biofilm performance. Thus, the intention of the present thesis was to identify and to address the different inhibition processes of Geobacter spp. dominated biofilm anodes in AD-MET combinations. Particular attention was devoted to the impact that planktonic methanogens, particles and dissolved components present in AD effluents may have on the activity, stability and microbial community of Geobacter spp. dominated biofilm anodes. This was achieved by investigating the effect of biofilm age, applied anode potentials as well as the role played by specific methanogens with different metabolisms on the activity, stability and microbial community of Geobacter spp. dominated biofilms. The results indicated that older Geobacter spp. dominated biofilm anodes (≥ 5-week-old) are far more active and stable than younger biofilms (≤ 3-week-old) in AD environments. Compared to high applied anode potential (0.4 V vs. Ag/AgCl sat. KCl), low applied anode potentials (-0.2 V to 0.2 V vs. Ag/AgCl sat. KCl) resulted in higher activities of Geobacter spp. dominated biofilm anodes in AD environments. Other results indicated that AD effluents dominated by strict acetoclastic methanogens (e.g., Methanothrix spp.) cause deterioration in biofilm stability and activity, in contrast to AD effluents dominated by hydrogenotrophic methanogens (e.g., Methanobacterium spp.). In conclusion, the thesis provides useful information for understanding and improving the performance of AD-MET combinations and ways to overcome the multiple hurdles encountered so far.:1 Introduction 12
1.1 Basics of microbiology of anaerobic digestion 12
1.1.1 Methanogens and their functionality 12
1.1.2 Anaerobic digestion - methanogens and bacteria 13
1.1.3 Microbial diversity in anaerobic digestion: focus on methanogens 14
1.1.4 Metabolism and syntrophy in anaerobic digestion 16
1.1.5 Parameters influencing anaerobic digestion 18
1.2 Microbial electrochemical technologies 19
1.2.1 Primary microbial electrochemical technologies - possible applications 19
1.2.2 Basic examples of primary microbial electrochemical technologies 21
1.2.3 Geobacter spp. as model electroactive microorganism in microbial electrochemical technologies 23
1.2.4 Modes of electron transfer between electroactive microorganisms and electrodes 24
1.2.5 Electrochemical characterization of biofilm anodes 27
1.3 Combination of anaerobic digestion and microbial electrochemical technologies 28
1.3.1 Benefits: biotechnological applications 28
1.3.2 Challenges: factors limiting the combination anaerobic digestion - microbial electrochemical technologies 29
1.4 Aims and thesis outline 30
2 Publication 1: Benefits of Age – Improved Resistance of Mature Electroactive Biofilm Anodes in Anaerobic Digestion 33
2.1 Supplementary information for “Benefits of Age – Improved Resistance of Mature Electroactive Biofilm Anodes in Anaerobic Digestion” 43
3 Publication 2: Combining Geobacter spp. dominated biofilms and anaerobic digestion effluents - the effect of effluent composition and electrode potential on biofilm activity and stability 55
3.1 Supplementary information for “Combining Geobacter spp. dominated biofilms and anaerobic digestion effluents - the effect of effluent composition and electrode potential on biofilm activity and stability” 67
4 Publication 3: A unified and simple medium for growing model methanogens 91
4.1 Supplementary information for “A unified and simple medium for growing model methanogens” 105
5 Publication 4: Effect of model methanogens on the activity, stability, and microbial community structure of Geobacter spp. dominated biofilm anodes 117
5.1 Supplementary information for “Effect of model methanogens on the activity, stability, and microbial community structure of Geobacter spp. dominated biofilm anodes” 153
6 Discussion 167
6.1 The older the biofilm, the higher its activity and resistance when combined with anaerobic digestion effluents 167
6.2 Low applied anode potential leads to high activity of Geobacter spp. dominated biofilm in anaerobic digestion environments. 168
6.3 The role of methanogens, abiotic particles, dissolved components in the combination anaerobic digestion – microbial electrochemical technologies 170
6.3.1 Abiotic particles, dissolved components present in anaerobic digestion effluents do not always interfere with the activity, stability and community of Geobacter spp. dominated biofilm anodes 170
6.3.2 The activity and community of Geobacter spp. dominated biofilm anodes in anaerobic digestion environments vary with the predominant group of methanogens 171
7 Conclusions and future prospects 173
8 References 176
9 Appendix 187
9.1 Author contribution statements of published articles 187
9.2 Curriculum vitae 193
9.3 List of publications and conference contributions 196
9.4 Acknowledgment 199
9.5 Declaration of authorship 201
| Identifer | oai:union.ndltd.org:DRESDEN/oai:qucosa:de:qucosa:85496 |
| Date | 19 May 2023 |
| Creators | Dzofou Ngoumelah, Daniel |
| Contributors | Universität Leipzig |
| Source Sets | Hochschulschriftenserver (HSSS) der SLUB Dresden |
| Language | English |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, doc-type:doctoralThesis, info:eu-repo/semantics/doctoralThesis, doc-type:Text |
| Rights | info:eu-repo/semantics/openAccess |
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