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Previous issue date: 2016-07-29 / FACEPE / Commiphora leptophloeos, popularmente conhecidas como Imburana de Sertão pertence à família Burseraceae e é tradicionalmente usada por tribos indígenas brasileiras para o tratamento de suas enfermidades. O presente trabalho objetivou analisar o perfil fitoquímico,isolamento de substância e as atividades: antioxidante e antimicrobiana dos extratos com diferentes polaridades de cascas de C. leptophloeos. Os compostos fenólicos foram identificados por HPLC e encontrados: os ácidos gálico, clorogênicos e protocatecóico. O H1NMR revelou a presença de hinoquinina, uma lignana bioativa caracterizada pela primeira vez nesta especie. No que se refere a atividade antimicrobiana, os valores de MIC de hinoquinina variou 0,0485-3,125 mg / mL em diferentes isolados clínicos de S. aureus, e mostraram uma atividade bactericida contra o MRSA isolados a partir de sangue (MMC 0,40 mg / mL) e secreção de pós-operatório (MMC = 3,125 mg / mL). Os extratos mostraram atividade antimicrobiana contra espécies de Mycobacterium, tais como M. smegmatis (CIM = 12,5 mg / mL) e de M. tuberculosis MIC (= 52 mg / mL). A toxicidade dos extratos também foi avaliada por testes in vitro com HC50 de 313 ± 0,5 ug / ml., Foi realizada a caracterização fitoquímica, mostrando compostos fenólicos dotados de alta atividade antioxidante em cinco extratos, como aquosa (CLAQE), metanol (CLMEE) clorofórmio (CLCLE), ciclo-hexano (CLCHE ) e acetato de etilo (CLAEE). revelando a presença de taninos, cumarinas, flavonóides e açúcares redutores. CLAQE apresentou os maiores teores de compostos fenólicos com 33,64 ± 0,5 mg GAE / g e flavonóides com 19,3 ± 0,70 mg QE / g para os teores de taninos CLCLE mostrou 9,70 ± 0,2 mg TAE / g, por cumarinas CLCHE conteúdo mostrou 1,14 ± 0,81 mg BZE / g e de açúcares solúveis CLMEE apresentada 53,30 g.100g-1mg. A capacidade antioxidante total (TAC%) todos os extratos mostraram uma actividade antioxidante significativa para ambas as concentrações de 1-10 mg / ml (≥100%, valor de p <0,001). Todos os extratos induziram baixos níveis de morte celular (viabilidade ≥ 80%) por período de 24 horas. Além disso, estes valores citotóxicos não foram correlacionadas com a fenóis (r = -0,15) ou flavonóides (r = -0,20). Portanto presentes resultados mostraram que C. leptophloeos possui alto potencial antioxidante bem como propriedades inibitórias contra a MRSA, bem como vários Gram-positivos, Gram-negativos e microorganismos fungos, Mycobacterium. / Commiphora leptophloeos, usually known as Imburana of Sertão belongs to the Burseraceae family and is traditionally used by Brazilian indigenous tribes for the treatment of their infirmities. Herein we performed full phytochemical characterization, showing its notable amounts of phenolics compounds endowed with high antioxidant activity in five extracts such as aqueous (CLAQE), methanolic (CLMEE) chloroform (CLCLE), cyclohexane (CLCHE) and ethyl acetate (CLAEE). C. leptophloeos phytochemical prospection revealed the presence of phenolic contents, tannins, coumarins, flavonoids and reducing sugars, secondary metabolites used by the plants for their defense. The qualitative phytochemical analysis by TLC of C. leptophloeos extracts detected the presence of phenolic compounds, flavonoids and reducing sugars. H1NMR analysis revealed the presence of hinokinin, a bioactive lignan characterized in this article. The phenolic compounds were identified by HPLC and three were described: Gallic, Chlorogenic and Protocatechuic acids. CLAQE presented the major contents of phenolic compounds with 33.64 ± 0.5 mg GAE/g and flavonoids with 19.3 ± 0.70 mg QE/g, for tannins contents CLCLE showed 9.70 ± 0.2 mg TAE/g, for coumarins content CLCHE showed 1.14 ± 0.81 mg BZE/g and for soluble sugars CLMEE presented 53.30 g.100g-1mg. In Total antioxidant capacity (%TAC) all extracts showed significant antioxidant activity for both concentrations of 1-10 mg/mL (≥100%, p-value <0.001). All the extracts induced low levels of cell death (viability ≥ 80%) within 24-hour period. In addition, cytotoxic results were not correlated with either phenols (r = -0.15) or flavonoids (r = -0.20). Altogether, we showed that the barks extracts from C. leptophloeos are antioxidants source being of potential use in preventing oxidative conditions. CLAQE presented the major contents of phenolic compounds with 33.64 ± 0.5 mg GAE/g and flavonoids with 19.3 ± 0.70 mg QE/g, for tannins contents CLCLE showed 9.70 ± 0.2 mg TAE/g, for coumarins content CLCHE showed 1.14 ± 0.81 mg BZE/g and for soluble sugars CLMEE presented 53.30 g.100g-1mg. In Total antioxidant capacity (%TAC) all extracts showed a significant antioxidant activity for both concentrations of 1-10 mg/mL (≥100%, p-value <0.001). The present findings showed that C. leptophloeos possesses inhibitory properties against MRSA as well as various Gram-positives, Gram-negatives and Fungi microorganisms, and should also be investigated for its potential against Mycobacterium. Furthermore, we have identified for the first time the presence of hinokinin in Commiphora genus. The MIC values of hinokinin ranged from 0.0485 to 3.125 mg/mL in different S. aureus clinical isolates, and showed a bactericidal activity against MRSA isolated from blood (MMC 0.40 mg/mL) and postoperative secretion (MMC = 3.125 mg/mL). C. leptophloeos extracts showed antimicrobial activity against Mycobacterium species such as M. smegmatis (MIC = 12.5 mg/mL) and M. tuberculosis (MIC = 52 mg/mL). Herein we also determined C. leptophloeos toxicity by HC50 tests in vitro with hemolytic activity detected of 313±0.5 μg/mL. All the extracts induced low levels of cell death (viability ≥ 80%) by 24-hour period. In addition, these cytotoxic values were not correlated with either phenols (r = -0.15) or flavonoids (r = -0.20).
| Identifer | oai:union.ndltd.org:IBICT/oai:repositorio.ufpe.br:123456789/27093 |
| Date | 29 July 2016 |
| Creators | PEREIRA, Aline de Paula Caetano |
| Contributors | http://lattes.cnpq.br/7863845087003953, CORREIA, Maria Tereza dos Santos |
| Publisher | Universidade Federal de Pernambuco, Programa de Pos Graduacao em Ciencias Biologicas, UFPE, Brasil |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/doctoralThesis |
| Source | reponame:Repositório Institucional da UFPE, instname:Universidade Federal de Pernambuco, instacron:UFPE |
| Rights | Attribution-NonCommercial-NoDerivs 3.0 Brazil, http://creativecommons.org/licenses/by-nc-nd/3.0/br/, info:eu-repo/semantics/openAccess |
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