Analysis of crystal structures of native antibodies bound to the hemagglutinin (HA) surface receptor of influenza have provided pertinent chemical information and metrics of the antibody-antigen interactions at the complimentary determining region (CDR) and receptor-binding domain (RBD) interface. We proposed that using this information, we could design a gold monolayer-protected cluster (AuMPC) functionalized with peptidic anti-HA paratopes and determine equilibrium rate constants of this particle-protein interaction. The peptidic ligands incorporate thiol surface modulators, polyethylene glycol spacing module, and specificity through a molecular recognition element (MRE). The MRE will be based upon the amino acid sequences of CDRs of specific antibodies to HA of pandemic influenza virus. Bio-layer interferometry experiments were conducted with these AuMPCs to determine equilibrium rate constants. The modular design of our ligands coupled with our platform made for an interdisciplinary and novel approach to nanoparticle biomimetics.
| Identifer | oai:union.ndltd.org:VANDERBILT/oai:VANDERBILTETD:etd-07272014-084905 |
| Date | 29 July 2014 |
| Creators | Bryant, Matthew Vann |
| Contributors | Dr. David Wright, Dr. Carmello Rizzo |
| Publisher | VANDERBILT |
| Source Sets | Vanderbilt University Theses |
| Language | English |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | http://etd.library.vanderbilt.edu/available/etd-07272014-084905/ |
| Rights | unrestricted, I hereby certify that, if appropriate, I have obtained and attached hereto a written permission statement from the owner(s) of each third party copyrighted matter to be included in my thesis, dissertation, or project report, allowing distribution as specified below. I certify that the version I submitted is the same as that approved by my advisory committee. I hereby grant to Vanderbilt University or its agents the non-exclusive license to archive and make accessible, under the conditions specified below, my thesis, dissertation, or project report in whole or in part in all forms of media, now or hereafter known. I retain all other ownership rights to the copyright of the thesis, dissertation or project report. I also retain the right to use in future works (such as articles or books) all or part of this thesis, dissertation, or project report. |
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