Primena sistema hitozan-jonska površinski aktivna materija za dobijanje mikrokapsula uljnog sadržaja / Application of chitosan-ionic surfactant system for the preparation of microcapsules with oil content

Description

<p style="text-align: justify;">Mikrokapsulacija je tehnika kojom se nestabilne, međusobno inkompatibilne i biolo&scaron;ki aktivne supstance prevode u stabilniji oblik, ili se omogućuje njihovo kontrolisano i ciljano oslobađanje. Osobine formiranih mikrokapsula, kinetika i mesto otpu&scaron;tanja inkapsuliranih materija zavise, pre svega, od njihovog omotača. Savremene tendencije razvoja prehrambenih proizvoda i proizvoda farmaceutske i kozmetičke industrije, su sve vi&scaron;e usmerene ka upotrebi prirodnih i biorazgradivih polimernih materija za formiranje omotača mikrokapsula.<br />Cilj ove disertacije je mogućnost primene hitozana, netoksičnog, biorazgradivog derivata hitina, kao materije omotača mikrokapsula sa uljnim sadržajem. S obzirom na njegovu slabu povr&scaron;insku aktivnost, istraživanja su usmerena na primenu interakcija hitozana sa suprotno naelektrisanim jonskim povr&scaron;inski aktivnim materijama (PAM) u vodenim rastvorima, kao mehanizmu za njegovo deponovanje na graničnoj povr&scaron;ini ulje/voda.<br />Primenom različitih metoda (tenziometrija, viskozimetrija, turbidimetrija, merenje elektroforetske pokretljivosti) interakcije hitozana sa natrijum-dodecil-sulfatom (SDS) i natrijum-lauriletar-sulfatom (SLES) su detaljno ispitane. Definisane su promene kako na granici faza, tako i unutar rastvora, a mehanizam formiranja kompleksa hitozan/PAM različitih osobina je u potpunosti razja&scaron;njen. Utvrđeno je da do formiranja stabilnog koacervata dolazi pri masenom odnosu hitozan:SLES 1:2 i hitozan:SDS 1:2.<br />Ispitivanje uticaja interakcije hitozan-PAM na osobine emulzionih sistema tipa ulje u vodi (veličina i raspodela veličina kapi, stabilnost) omogućilo je odabir hitozan-SLES sistema kao omotača pogodnog za dobijanje mikrokapsula uljnog sadržaja. Na osnovu ovih rezulatata kao uljna faza odabrani su trigliceridi srednje dužine ugljovodoničnih lanca (TSDL). Su&scaron;enjem emulzija primenom spray drying postupka dobijene se mikrokapsule uljnog sadržaja sa vitaminom E i ispitan je uticaj umreživača na njihove osobine. Karakterizacijom dobijenih mikrokapsula (određivanje sadržaja vlage, ispitivanje morfologije povr&scaron;ine, efiksanost inkapsulacije vitamina E, kinetika otpu&scaron;tanja vitamina E u in vitro uslovima) zaključeno je da na osobine mikrokapsula utiče vrsta i koncentracija umreživača. Mikrokapsule čiji omotač nije umrežen pokazale su najbolje karakteristike.</p> / <p>Microencapsulation is a technique that unstable, incompatible and biologically active substances converted to a more stable form, or allow their controlled and targeted release. The properties of formed microcapsules, kinetics and the place of release of encapsulated substances, primarly depend on their shell characteristic. Modern trends in the development of food products and products of the pharmaceutical and cosmetic industries are increasingly focused on the use of natural and biodegradable polymeric materials for coatings.<br />The aim of this dissertation is to investigate the possibility of using chitosan, non-toxic and biodegradable chitin derivative, as a shell material of microcapsules with oil content. Due to its low surface activity, the research is focused on the utilization of chitosan interactions with opositly charged ionic surfactants (sodium dodecyl sulfate (SDS) and sodium lauryl ether sulfate (SLES)) in aqueous solutions as mechanisms for its depositing at the oil/water interface.<br />Based on detailed investigation of interactions in the chitosan-ionic surfactant systems using different methods (tensiometry, viscometry, turbidimetry, measurement of electrophoretic mobility), changes have been defined both at the interface and within the bulk, as well as the mechanism of formation of the coacervate phase. It has been determined that at chitosan:SLES and chitosan:SDS mass ratio of 1:2 stabile coacervate were formed.<br />An investigation of the influence of interaction on the properties of oil-in-water emulsion systems (size and distribution of droplet size, stability) enabled the chitosan-SLES system to be selected as a shell suitable for obtaining microcapsules of the oil content. Also, based on these results, medium-chain triglycerides were selected as the oil phase of the emulsion.<br />Microcapsules with vitamin E were obtained by spray drying of emulsions stabilized with chitosan/SLES complex. The influence of the crosslinker on the properties of microcapsules was investigated. Characterization of obtained microcapsules (moisture content determination, investigation of the surface morphology, efficiency of the vitamin E encapsulation, release in vitro kinetics of vitamin E) showed that type and concentration of crosslinking agents had influences the properties of microcapsules. Microcapsules without crosslinking agents have the most suitable characteristics.</p>

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1. https://www.cris.uns.ac.rs/DownloadFileServlet/Disertacija155428632085927.pdf?controlNumber=(BISIS)110573&fileName=155428632085927.pdf&id=12747&source=NDLTD&language=en
2. https://www.cris.uns.ac.rs/record.jsf?recordId=110573&source=NDLTD&language=en

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Identifer	oai:union.ndltd.org:uns.ac.rs/oai:CRISUNS:(BISIS)110573
Date	05 July 2019
Creators	Milinković Budinčić Jelena
Contributors	Petrović Lidija, Katona Jaroslav, Fraj Jadranka, Đekić Ljiljana
Publisher	Univerzitet u Novom Sadu, Tehnološki fakultet Novi Sad, University of Novi Sad, Faculty of Technology at Novi Sad
Source Sets	University of Novi Sad
Language	Serbian
Detected Language	English
Type	PhD thesis