Dietary cyclopropenoid fatty acids (CPFA) have long
been known to cause pronounced physiological disorders in
both farm and laboratory animals. Past work has shown
CPFA to be a powerful promoter of carcinogenesis in trout,
rats and mice. Sterculic and malvalic acids (CPFA's) are
found in seed lipids of plants from the order Malvales.
Two members of this order are cotton and kapok, both of
which are used extensively as cooking oils for human
consumption.
Present chemical and instrumental methods of analysis
for CPFA are effective only at CPFA levels above 0.1%, and
accurate only at levels above 1%. A more sensitive method
of analysis was developed exploiting recent technological
advances in glass capillary gas chromatography (GCGC). By
the use of cold on-column injection, and positioning of
the column at the base of the detector flame, this method
eliminates two problem areas found in other GC methods.
The other common component involved in CPFA decomposition,
during GC analysis, is the column. Vitrious silica columns with an inert stationary phase, SE-30, were shown
to separate the highly reactive CPFA without
decomposition. Proof of stability during analysis was
obtained by changing the variables of relative time on
column and column temperature. This method not only
allows individual determination of sterculic and malvalic
acid concentrations, it is rapid, accurate (to the
70 parts per million range), and is superior to other
instrumental and chemical methods.
The CPFA concentration for okra, hollyhock, cheese
weed, seashore mallow, kapok, and white cap cottonseed oil
are as follows: 0.3% to 0.92%, 0.33%, 2.6%, 2.6%, 12.8%,
and 75ppm, respectively. The two different values for
okra were found because seeds from two different growing
seasons were analyzed. No CPFA could be detected in Diet
Imperial Margarine, raw cocoa beans, cocoa butter or
Lucca's winterized cottonseed oil. / Graduation date: 1987
| Identifer | oai:union.ndltd.org:ORGSU/oai:ir.library.oregonstate.edu:1957/27215 |
| Date | 09 March 1987 |
| Creators | Ryan, Daberath |
| Contributors | Pawlowski, Norman E. |
| Source Sets | Oregon State University |
| Language | en_US |
| Detected Language | English |
| Type | Thesis/Dissertation |
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