Return to search

Development of a Molecular Marker to Track APA G40199 Introgression in Common Bean for Bruchid Resistance

In common bean (Phaseolus vulgaris), the main seed storage pests are the bruchid beetles. Damage done to the seed by the larvae has a large impact on seed quality and yield. Arcelin (ARC), phytohaemagglutinin (PHA), and α-amylase inhibitor (α-AI) are linked seed storage proteins that form the APA locus on chromosome Pv04 and are associated with resistance. A major breeding objective is to introduce bruchid resistance into common bean from a resistant tepary genotype, G40199, by introgressing the resistant APA locus into susceptible common bean backgrounds. Here we developed a molecular marker that tracks the introgression. A set of PCR primers to the α-amylase inhibitor locus amplified a DNA fragment that showed a 45 base pair insertion in the middle of a lectin Leg_b domain. This enhanced locus characterization and insertion/deletion marker may preclude the need for bruchid resistance screening early in the breeding. / United States. Agency for International Development / United States. Global Hunger and Food Security Initiative (Cooperative Agreement No. EDH-A-00-07-00005-00)

Identiferoai:union.ndltd.org:ndsu.edu/oai:library.ndsu.edu:10365/29300
Date January 2018
CreatorsMazaheri, Lucy
PublisherNorth Dakota State University
Source SetsNorth Dakota State University
Detected LanguageEnglish
Typetext/thesis
Formatapplication/pdf
RightsNDSU policy 190.6.2, https://www.ndsu.edu/fileadmin/policy/190.pdf

Page generated in 0.0014 seconds