Carvajal-Aldaz, Diana Gabriela, B.S. Food Science, Zamorano University, 2007
Master of Science, Fall Commencement, 2012
Major: Food Science
Inhibition of Adipocyte Differentiation in 3T3-L1 Cell Line by Quercetin or Isorhamnetin
Thesis directed by Professor Jack N. Losso
Pages in thesis, 43. Words in abstract, 281.
ABSTRACT
Obesity has become a major health problem worldwide. Obesity increases the risk of hypertension, diabetes, and certain types of cancer. Quercetin is a bioactive compound widely found in a variety of foods that are consumed daily. Isorhamnetin is a bioactive compound found in some foods and also is a quercetin metabolite. Many studies have reported the anti-obesity and anti-inflammation properties of quercetin and isorhamnetin. The objective of this study was to test the effect of quercetin or isorhamnetin at physiological and supraphysiological concentrations on the inhibition of the differentiation process of 3T3-L1 pre-adipocyte to adipocyte. Cell viability results demonstrated no significant difference (P > 0.05) between non differentiated cells, control and quercetin or isorhamnetin treated cells. During adipocyte differentiation for 8 days in the presence of quercetin or isorhamnetin, cell viability was above 94.84% and 97.63%, respectively. Red oil O staining assay was performed in order to evaluate the inhibitory effect of quercetin or isorhamnetin on cytoplasmic lipid droplet accumulation. Significant differences (P < 0.05) were reported. Isorhamnetin was more effective than quercetin in inhibiting cytoplasmic lipid droplet accumulation. Neither quercetin nor isorhamnetin had an effect on the expression of macrophage chemoattractant protein -1 (MCP-1). CCAAT/enhancer binding protein α (C/EBP-α) was down-regulated by quercetin or isorhamnetin. Compared to control quercetin decreased PPAR-γ 1 and 2 expressions by 45.03 ± 3.17% and 27.58 ± 12.39%, while isorhamnetin decreased PPAR-γ 1 and 2 expressions by 41.48 ± 9.51% and 2.01 ± 32.46%, respectively. β-catenin was not dose dependent either for quercetin or isorhamnetin and did not follow a specific trend. Taken together, our data indicate that isorhamnetin more than quercetin can exert potential anti-obesity effects by inhibiting differentiation of pre-adipocytes at physiological concentrations.
| Identifer | oai:union.ndltd.org:LSU/oai:etd.lsu.edu:etd-11092012-160754 |
| Date | 15 November 2012 |
| Creators | Carvajal-Aldaz, Diana Gabriela |
| Contributors | Enright, Frederick, Finley, John, Losso, Jack |
| Publisher | LSU |
| Source Sets | Louisiana State University |
| Language | English |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | http://etd.lsu.edu/docs/available/etd-11092012-160754/ |
| Rights | unrestricted, I hereby certify that, if appropriate, I have obtained and attached herein a written permission statement from the owner(s) of each third party copyrighted matter to be included in my thesis, dissertation, or project report, allowing distribution as specified below. I certify that the version I submitted is the same as that approved by my advisory committee. I hereby grant to LSU or its agents the non-exclusive license to archive and make accessible, under the conditions specified below and in appropriate University policies, my thesis, dissertation, or project report in whole or in part in all forms of media, now or hereafter known. I retain all other ownership rights to the copyright of the thesis, dissertation or project report. I also retain the right to use in future works (such as articles or books) all or part of this thesis, dissertation, or project report. |
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