M. Tech. / The Driver Resonator, manufactured by Makulu Mutis (Closed Corporation), is a registered electro-medical device used in practise by a number of medical and allied health professionals. The Driver Resonator was developed to assist with energy flow regulation, the elimination and/or reduction of viruses, bacteria and other pathogens, detoxification and pain management by means of an electrical impulse to the human body. There is currently no evidence available to determine the direct effect, if any, of the frequencies of the Driver Resonator on specific pathogens. A growth curve was constructed to determine the three different phases of growth for Candida albicans. Using the data obtained from the growth curve, C. albicans’ cells were harvested during the three selected growth phases and treated with the Driver Resonator at a prescribed frequency of 385 kHertz. The cells were treated consecutively for ten minutes at time intervals of 0, 2 and 4 hours and samples taken after each treatment to determine the amount of viable cells after treatment. Cells were treated in the presence of nutrients (growth media) and in the absence of nutrients (phosphate buffered saline) to investigate the possible effect the nutrients might have on the cells ability to recover after treatment. All experiments were performed in triplicate and included a set of untreated cells (positive control) to evaluate the effect of treatment. From the results it can be deduced that the Driver Resonator had no effect on C. albicans’ cell viability and recovery in the absence or presence of nutrients when cells are in the mid-exponential, late-exponential and stationary phases of growth.
| Identifer | oai:union.ndltd.org:netd.ac.za/oai:union.ndltd.org:uj/uj:6725 |
| Date | 31 March 2010 |
| Creators | Jansen van Vuuren, Audrey |
| Source Sets | South African National ETD Portal |
| Detected Language | English |
| Type | Thesis |
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