The lipid composition of swine is characteristic of the species
and is also a reflection of their dietary history. Other investigators
have established that the physical properties and composition of
swine depot fat may be altered by variation of the dietary lipid.
Dietary regimens employing vegetable oil-bearing materials and
their effect on meat quality has received the attention of animal
nutritionists for a considerable period. Less is known about the
nutritive value of the more highly unsaturated lipids, such as fish
oils, and their effect on the lipid composition of swine tissues.
Even less is known about the fate of oxidized fish oil lipids in
swine rations.
This research had for its purpose the investigation of the fate
in swine of the dietary marine lipid, menhaden oil, and its effect on
composition of depot lipids. The state of autoxidation of the oil and
the effect of certain antioxidants on the fatty acid composition of
swine was determined.
Growth studies on swine were made using menhaden oil of
various oxidation states as the dietary lipid. The effect of antioxidants,
∝-tocopherol acetate and ethoxyquin, was also investigated.
At the conclusion of the diet trials, tissue samples, representing
the five dietary regimens, were removed for analysis. The fatty
tissues examined were outer back fat, inner back fat, and kidney
fat. Samples of liver tissue for lipid analysis were also taken. The
lipids from the respective tissues were extracted and interesterified
with methanol to yield the methyl esters of the fatty acids. Hydrogenation
of the unsaturated methyl esters for chain length confirmation
was carried out.
Qualitative and quantitative gas-liquid chromatographic analysis
of the unhydrogenated and hydrogenated methyl esters of the fatty
acids were performed on diethylene glycol succinate column.
The results of these investigations showed that a particular
dietary fatty acid can be selectively deposited in animal tissues.
Long chain polyunsaturated fatty acids of menhaden oil, such as
20:5, 22:5, 22:6 were sparsely deposited in adipose tissues. The
depot fat composition showed a mixture of characteristic menhaden
oil fatty acids with the typical fat synthesized by swine.
Fatty acid composition of tissue from swine fed oxidized menhaden
oil with and without antioxidants showed very similar fatty
acid composition as those fed fresh menhaden oil. The results of
this investigation supported the beneficial effect of vitamin E
(∝-tocopherol acetate) and ethoxyquin as in vivo antioxidants. / Graduation date: 1964
| Identifer | oai:union.ndltd.org:ORGSU/oai:ir.library.oregonstate.edu:1957/26683 |
| Date | 27 February 1964 |
| Creators | Huang, Anita Wong |
| Contributors | Sinnhuber, Russell O. |
| Source Sets | Oregon State University |
| Language | en_US |
| Detected Language | English |
| Type | Thesis/Dissertation |
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