Violacein is a purple pigment typically produced by species of Chromobacterium and Janthinobacterium. A soil isolate, identified as Janthinobacterium, was studied. Maximal pigmentation occurred at 250C under aerobic conditions in the Keeble and Cross medium. Intracellular pigment was shown to be located in the cell membrane. Comparision of pigment production and growth curves indicated that violacein is synthesized in the cell and released into the environment possibly as a result of cell lysis. Extracellular pigment is water soluble, makes up 60% of the total pigment and shows a blue shift when compared to solvent extracted pigment. Results from purification indicated that the pigment is non-covalently bound to a small protein and aggregated into a larger molecule.
| Identifer | oai:union.ndltd.org:unt.edu/info:ark/67531/metadc798326 |
| Date | 05 1900 |
| Creators | Lin, Ying-Chuan |
| Publisher | University of North Texas |
| Source Sets | University of North Texas |
| Language | English |
| Detected Language | English |
| Type | Thesis or Dissertation |
| Format | vii, 67 leaves : ill., Text |
| Rights | Public, Lin, Ying-Chuan, Copyright, Copyright is held by the author, unless otherwise noted. All rights |
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