Immobilization of proteins by covalent coupling to polymeric materials offers numerous
excellent advantages for various applications, however, it is usually limited by coupling
strategies, which are often too expensive or complex. In this study, an electron-beambased
process for covalent coupling of the model protein bovine serum albumin (BSA)
onto polyvinylidene fluoride (PVDF) flat sheet membranes was investigated. Immobilization
can be performed in a clean, fast, and continuous mode of operation without any additional
chemicals involved. Using the Design of Experiments (DoE) approach, nine process factors
were investigated for their influence on graft yield and homogeneity. The parameters could
be reduced to only four highly significant factors: BSA concentration, impregnation
method, impregnation time, and electron beam irradiation dose. Subsequently,
optimization of the process was performed using the Response Surface Methodology
(RSM). A one-step method was developed, resulting in a high BSA grafting yield of
955 mgm−2 and a relative standard deviation of 3.6%. High efficiency was demonstrated
by reusing the impregnation solution five times consecutively without reducing the final
BSA grafting yield. Comprehensive characterization was conducted by X-ray
photoelectron spectroscopy (XPS), Fourier-transform infrared spectroscopy (FTIR), and
measurements of zeta potential, contact angle and surface free energy, as well as filtration
performance. In addition, mechanical properties and morphology were examined using
mercury porosimetry, tensile testing, and scanning electron microscopy (SEM).
| Identifer | oai:union.ndltd.org:DRESDEN/oai:qucosa:de:qucosa:84528 |
| Date | 03 April 2023 |
| Creators | Schmidt, Martin, Abdul Latif, Amira, Prager, Andrea, Gläser, Roger, Schulze, Agnes |
| Publisher | Frontiers Research Foundation |
| Source Sets | Hochschulschriftenserver (HSSS) der SLUB Dresden |
| Language | English |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, doc-type:article, info:eu-repo/semantics/article, doc-type:Text |
| Rights | info:eu-repo/semantics/openAccess |
| Relation | 2296-2646, 804698 |
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