I have measured the metabolism of tRNA$ sp{ rm Asn}$ in Friend cells undergoing erythroid differentiation, using an assay exploiting the tRNA$ sp{ rm Asn}$ gene's ability to specifically hybridize tRNA$ sp{ rm Asn}$. The concentrations of tRNA$ sp{ rm Asn}$ measured as a proportion of the total tRNA population (relative concentration) decreased until day 3 post-induction, and then increased on day 4. The relative concentrations of tRNA$ sp{ rm Asn}$ are influenced by tRNA$ sp{ rm Asn}$ having lower turnover rate in comparison to the total tRNA population, and by the relative rate that labelled, newly synthesized tRNA$ sp{ rm Asn}$ accumulates in vivo. The rate of tRNA$ sp{ rm Asn}$ synthesis in nuclei isolated from Friend cells at different times during differentiation also fluctuates. This fluctuation may reflect changes in RNA polymerase III activity in these isolated nuclei.
Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.60725 |
Date | January 1992 |
Creators | Miller, Harvey |
Publisher | McGill University |
Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
Language | English |
Detected Language | English |
Type | Electronic Thesis or Dissertation |
Format | application/pdf |
Coverage | Master of Science (Department of Biology.) |
Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
Relation | alephsysno: 001287765, proquestno: AAIMM74587, Theses scanned by UMI/ProQuest. |
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