Impairment of the intestinal microcirculation (IMC) plays a critical role in the
pathogenesis of sepsis. Consequently, the protection of the IMC represents a pivotal
therapeutic target in severe sepsis. The aim of this study was to examine the effects of
endocannabinoid system modulation on the IMC. Experimental animals groups were:
control, endotoxemic animals (lipopolysaccharide; LPS), LPS + CB1R agonist, LPS +
CB1R antagonist, LPS + CB1R agonist + CB1R antagonist, LPS + CB2R agonist, LPS +
CB2R antagonist, LPS + CB2R agonist + CB2R antagonist, LPS + cannabinoid
degradation enzyme inhibitor and LPS + enzyme inhibitor + CB2R antagonist.
Endotoxemia significantly increased leukocyte adhesion in intestinal submucosal venules, and significantly reduced capillary perfusion of the muscular and mucosal layers of the intestinal wall. In acute experimental endotoxemia, IMC was significantly improved (by reducing leukocyte adhesion and increasing capillary perfusion) with CB1R inhibition or CB2R activation or inhibition of endocannabinoid degradation.
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:NSHD.ca#10222/15187 |
| Date | 25 July 2012 |
| Creators | Kianian, Mandana |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
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