In this study, a LC/MS/MS method for the determination of ampicillin antibiotic in fish muscle tissue was developed and accredited according to Commission Decision 2002/657/EC. The metabolism of ampicillin in tilapia was them studied in serum, liver and muscle.
The homogenized fish tissue was first extracted with MeOH-H2O(4:1), C18 sorbent was added to remove lipids and impurities, the extract was then evaporated to dryness with a steam of nitrogen gas at 38 ¢XC. The residue was redissolved with H2O, filtered and analyzed by LC/MS/MS equipped with an Agilient HC-C18(5£gm, 150mm ¡Ñ4.6mm), the mobile phase A was 10mM ammonium acetate containing 0.1% formic acid, while the mobile phase B was methanol. The determination of ampicillin was performed with electrospray ionization-tandem mass spectrometry in positive mode using multiple reation monitoring(MRM) for detection. Average recoveries were 81¡V86%, the limit of detection was 6.00 £gg kg-1¡Adecision limit(CC£\) of ampicillin in fish muscle sample was 63.65 ¡Ó 7.99 £gg kg-1.
In the metabolism study, the oral administered dose to talipia was 20 mg/kg¡DBW. The maximum concentration of ampicillin in each tissues was obserned at 0.5 hour after oral administration, the maximum concentration in serum, liver and muscle was 27.53 mg L-1, 66.75 mg kg-1 and 1.33 mg kg-1, respectively. The concentration of ampicillin in muscle was 0.04 mg kg-1 24 hours after oral administration, which is lower than the 0.05 mg kg-1 MRL value of European Union resolutions. No residual ampicillin was detected in tilapia 48 hours after oral treatment, which conformed to the drug regulations for aquaculture ainmals in Taiwan.
| Identifer | oai:union.ndltd.org:NSYSU/oai:NSYSU:etd-0824111-010108 |
| Date | 24 August 2011 |
| Creators | Lin, You-nan |
| Contributors | Jung-Hui Chen, Wei-Hsien Wang, Chi-Hsin Hsu |
| Publisher | NSYSU |
| Source Sets | NSYSU Electronic Thesis and Dissertation Archive |
| Language | Cholon |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0824111-010108 |
| Rights | user_define, Copyright information available at source archive |
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