<p>Chicken fetuses exposed to chronic hypoxia suffer from growth retardation and</p><p>induces an overall sympathetic activity, including elevation of the concentration</p><p>of circulating catecholamines. Simultaneously, hypoxic fetuses display a</p><p>lowered β-adrenoreceptor (βAR) density in myocardial tissue. In vertebrates,</p><p>β1AR and β2AR are the most important signalling pathways for acute elevation</p><p>of cardiac performance. The aim of this study was to see how chronic hypoxia</p><p>affects the level of messenger RNA (mRNA) for the β1AR in the fetal chicken</p><p>heart at different developmental ages. The broiler chicken is a suitable model</p><p>organism for studying the progression of heart failure because the fast growth</p><p>rate requires a large increase in blood perfusion at the end of fetal development.</p><p>The β1AR sequence of the broiler chicken is 1587 bp and located on</p><p>chromosome 6. When running a PCR for quantification of the sequence,</p><p>primers for almost the whole sequence failed (1404 bp) and so did primers of</p><p>1193 bp; instead primers of 692 bp of the sequence were used and made</p><p>quantification possible. Similar results were obtained from both the heart and</p><p>liver of day 15 fetal chickens. The PCR product was cloned into a TOPO vector</p><p>and sent for sequencing, to enable the making of a probe for a northern blot</p><p>analysis of the mRNA in the fetal chicken hearts.</p>
| Identifer | oai:union.ndltd.org:UPSALLA/oai:DiVA.org:liu-9819 |
| Date | January 2006 |
| Creators | Hedlund, Sebastian |
| Publisher | Linköping University, The Department of Physics, Chemistry and Biology, Institutionen för fysik, kemi och biologi |
| Source Sets | DiVA Archive at Upsalla University |
| Language | English |
| Detected Language | English |
| Type | Student thesis, text |
| Relation | Linköping Studies in Science and Technology. Thesis, 0280-7971 ; |
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