Reported here is an investigation to determine if azinphos-methyl (AZM), an
organophosphate insecticide, adducts to hemoglobin, and if so, whether the
hemoglobin adduct could be used as a quantitative marker of occupational AZM
exposure. We hypothesized that AZM, or a metabolite of AZM, binds to hemoglobin
in erythrocytes forming an adducted protein. We administered radiolabled AZM to
rats and found a stable, dose-dependent association of radioactivity with hemoglobin.
The decline in hemoglobin-associated radioactivity followed the expected kinetics of
erythrocyte turnover in rats. We examined hemoglobin isolated from these rats by
high-pressure liquid chromatography, liquid scintillation counting, and electrospray
ionization mass spectrometry. These analyses provided evidence of AZM or an AZM
metabolite binding to one of the beta proteins of hemoglobin. In vitro incubation of
AZM with hemoglobin in a liver microsome system indicated an AZM adduct to
heme. Further research is necessary to fully characterize the adduct and determine
whether this biomarker will be useful for monitoring human exposure to AZM. / Graduation date: 2000
| Identifer | oai:union.ndltd.org:ORGSU/oai:ir.library.oregonstate.edu:1957/33332 |
| Date | 05 May 2000 |
| Creators | Bailey, Bonnie J. |
| Contributors | Jenkins, Jeffrey |
| Source Sets | Oregon State University |
| Language | en_US |
| Detected Language | English |
| Type | Thesis/Dissertation |
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