Chlorpheniramine, a reversible competitive inhibitor at the H1-receptor that has demonstrated potent, long-lasting antihistaminic activity with only mild side-effects, has been a popular choice for the treatment of allergic conditions and is a common component of cold/cough preparations. A number of groups have studied the pharmacokinetics of chlorpheniramine since its development, but results have been conflicting. Moreover, most of the pharmacokinetic studies had only involved analysis of the parent compound and excluded pharmacologically active metabolites. / As part of a new pharmacokinetic study of chlorpheniramine, an enantiomeric method to resolve chlorpheniramine from its N-demethylated metabolites and chlorpheniramine N-oxide was required. The use of high performance liquid chromatography (HPLC) with the amylose tris(3,5-dimethylphenylcarbamate) chiral stationary phase (AD-CSP) failed to resolve the enantiomers of chlorpheniramine and its metabolites. Capillary electrophoresis was used to screen a number of chiral selectors including hydroxypropyl beta-cyclodextrin, sulfated beta-cyclodextrin and carboxymethyl beta-cyclodextrin. The resolution of the enantiomers of chlorpheniramine and its metabolites was achieved using carboxymethyl beta-cyclodextrin obtained from Wacker Chemie, Munich, Germany. Detection limits of chlorpheniramine down to 200 ng/mL were achieved by concentrating samples and using sample stacking methods.
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.20868 |
| Date | January 1998 |
| Creators | Soo, Evelyn Chun-Yin. |
| Contributors | Wainer, I. W. (advisor), Kazlauskas, R. (advisor) |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Master of Science (Department of Chemistry.) |
| Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
| Relation | alephsysno: 001641729, proquestno: MQ44284, Theses scanned by UMI/ProQuest. |
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