by Ka-ming Pang. / Thesis (M.Phil.)--Chinese University of Hong Kong, 1990. / Bibliography: leaves 95-100. / Abstract --- p.i / Introduction --- p.1 / Literature review --- p.2 / Chapter 1. --- Mode of Respiration in Enteric Bacteria --- p.2 / Chapter 2. --- Nitrate Respiration --- p.4 / Chapter 3 . --- Biochemistry of Nitrate Reductase --- p.7 / Chapter 4 . --- Regulation of Nitrate Reductase --- p.8 / Chapter 5. --- Genetics and Regulation of chlC (nitrate Reductase) --- p.10 / Chapter 6. --- chlC in Salmonella typhimurium --- p.14 / Chapter 7. --- Purpose of study --- p.16 / Materials and Methods / Chapter 1 . --- Strains --- p.18 / Chapter 2. --- Media --- p.18 / Chapter 3 . --- Solution --- p.20 / Chapter 4. --- β-galactosidase assay --- p.23 / Chapter 5. --- Preparation of chromosome DNA --- p.24 / Chapter 6. --- Small scale plasmid preparation --- p.25 / Chapter 7. --- Large scale plasmid preparation --- p.26 / Chapter 8. --- Preparation of lambda DNA --- p.27 / Chapter 9. --- Preparation of M13 ssDNA for sequencing --- p.30 / Chapter 10. --- Digestion of DNA with restriction enzymes --- p.31 / Chapter 11 . --- Agarose gel electrophoresis --- p.31 / Chapter 12. --- Ligation --- p.31 / Chapter 13. --- Transformation --- p.32 / Chapter 14. --- Direct gel test and C-test of ssDNA of M13 clones --- p.34 / Chapter 15. --- DNA sequencing --- p.34 / Results / Chapter 1 . --- β-galactosidase assay of HSK1001 --- p.37 / Chapter 2. --- Preparation of HSK1001 DNA --- p.37 / Chapter 3. --- Sau3A partial digestion of HSK1001 chromosomal DNA --- p.37 / Chapter 4 . --- Ligation of HSK1001 DNA to lambda EMBL3 --- p.40 / Chapter 5. --- In vitro packaging and screening of lambda clones --- p.40 / Chapter 6. --- Restriction mapping of lambda clones --- p.43 / Chapter 6.1 --- Restriction mapping of HSK4001 to HSK4006 --- p.43 / Chapter 6.2 --- Orientation of Mu dA fusion clones --- p.43 / Chapter 7 . --- "Sub-cloning of SalI-HindIII fragments of HSK4002 HSK4003, HSK4005 and HSK4006 into pFZYl" --- p.66 / Chapter 8. --- Sub-cloning of 2.4 kb HindIII fragment of HSK4005 and HSK4006 into pFZY1 --- p.67 / Chapter 9. --- Sub-cloning of 2.4 kb HindIII fragment of HSK4006 into M13mpl9 --- p.67 / Chapter 10. --- Sub-cloning of 2.4 kb HindIII fragment of HSK4005 into M13mp19 --- p.69 / Chapter 11. --- Sub-cloning of 2.5 kb SalI-HindIII fragment of HSK4006 into M13mpl8 --- p.72 / Chapter 12. --- Sub-cloning of 2.2 kb SalI-HindIII fragment of HSK4005 into M13mp19 --- p.72 / Chapter 13. --- Complementation test of M13 clones --- p.74 / Chapter 14. --- Sub-cloning of 4.3 kb HindIII-EcoRI fragment and 3.8 kb EcoRI-BamHI fragments of HSK4006 into M13 mp18 and mp19 --- p.74 / Chapter 15. --- DNA sequences of M13 clones --- p.77 / Dicussion / Chapter 1 . --- Cloning of Mu dA operon fusion to EMBL3 vector --- p.88 / Chapter 2 . --- Sequences of SalI-HindIII fragments of HSK4005 to HSK4006 --- p.92 / Reference --- p.95
| Identifer | oai:union.ndltd.org:cuhk.edu.hk/oai:cuhk-dr:cuhk_318605 |
| Date | January 1990 |
| Contributors | Pang, Ka-ming., Chinese University of Hong Kong Graduate School. Division of Biology. |
| Publisher | Chinese University of Hong Kong |
| Source Sets | The Chinese University of Hong Kong |
| Language | English |
| Detected Language | English |
| Type | Text, bibliography |
| Format | print, ii, 100 leaves : ill. ; 30 cm. |
| Rights | Use of this resource is governed by the terms and conditions of the Creative Commons “Attribution-NonCommercial-NoDerivatives 4.0 International” License (http://creativecommons.org/licenses/by-nc-nd/4.0/) |
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