The potential for an electrokinetically driven, FRET-based microfluidic biosensor for SNP discrimination has been explored. The method functionalized the glass wall of a PDMS/glass microfluidics channel with multidentate thiol ligands to noncovalently immobilize MPA capped CdSe/ZnS QDs. Single stranded probe DNA could then be immobilized to QDs and target material could be delivered electrokinetically to the sensing surface. SNP discrimination could then occur by manipulation of shear, electrical and thermal effects derived from the applied voltage. The stability of immobilized QDs was investigated by EOF experiments that applied 500 V and 100 V voltages for 10 minutes to initiate electrokintetic flow. Fluorescence intensity measurements showed nearly complete removal of QDs from slides when compared with controls at both voltages. Pressure driven flow experiments demonstrated reduced dissociation of immobilized of QDs compared to channels exposed to EOF. A covalent approach is likely necessary to ensure stability of immobilized QDs during EOF.
| Identifer | oai:union.ndltd.org:TORONTO/oai:tspace.library.utoronto.ca:1807/17498 |
| Date | 30 July 2009 |
| Creators | Cauchi, Jonathan |
| Contributors | Krull, Ulrich Jorg |
| Source Sets | University of Toronto |
| Language | en_ca |
| Detected Language | English |
| Type | Thesis |
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