Recent proteome studies have discovered the presence of heat shock protein 10 (HSP-10) as an immunosuppressant in ovarian cancer patients. Due to the severity of ovarian cancer, the development of highly sensitive techniques for the early detection of this cancer is well in demand. In this manuscript, the thickness shear mode (TSM) acoustic wave biosensor will be used for the real-time and label-free detection of HSP-10 in buffer. The TSM sensitivity for HSP-10 is evaluated based on resonance frequency shifts generated by the biosensor. A nucleic acid aptamer, which is specifically engineered by in vitro selection to target HSP-10, is employed as the biosensing element of the biosensor. Alkylthiol-based self-assembling monolayers (SAMs), composed of various linker/diluent molar ratios, are used to immobilize the aptamer onto gold-coated piezoelectric quartz substrates. The TSM biosensing properties for avidin-biotin interactions are also evaluated in order to assess the biosensor response to HSP-10 protein-aptamer interaction.
| Identifer | oai:union.ndltd.org:TORONTO/oai:tspace.library.utoronto.ca:1807/24636 |
| Date | 28 July 2010 |
| Creators | Saoud, Marwan |
| Contributors | Thompson, Michael |
| Source Sets | University of Toronto |
| Language | en_ca |
| Detected Language | English |
| Type | Thesis |
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