Bioinformatic methods were applied to address biological questions. Two new eFP browser web
tools were constructed for the intuitive visualization of data from large-scale data sets. In
addition, a predicted interactome was constructed for Arabidopsis thaliana and validated using a
gene coexpression analysis. The Arabidopsis Interactions Viewer was created to enable access to
and visualization of predicted and confirmed interactions in the Arabidopsis interactome.
In a separate analysis short sequence matches were identified between introns and coding
sequences in several model systems including Arabidopsis, human, C. elegans and 12 Drosophila
species. Several hundred to thousands of matches were found near each other in terms of
chromosomal location, and were termed Proximal Intron N-mer (PIN) matches. Sequence
matches were conserved between 11 Drosophila species and D. melanogaster, suggesting a
potential functional role. Novel plasmids were designed to test whether PIN matches are
functional in vivo.
| Identifer | oai:union.ndltd.org:TORONTO/oai:tspace.library.utoronto.ca:1807/11130 |
| Date | 30 July 2008 |
| Creators | Ammar, Ron |
| Contributors | Provart, Nicholas |
| Source Sets | University of Toronto |
| Language | en_ca |
| Detected Language | English |
| Type | Thesis, Software |
| Format | 73966 bytes, 4374729 bytes, application/octet-stream, application/pdf |
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