The family of 14-3-3 proteins is crucial for various physiological cellular processes such as signaling, cell growth, division, differentiation, and apoptosis. One of the 14-3-3 proteins members, 14-3-3 gamma, is abundantly expressed in brain and had been detected in the cerebrospinal fluid of
patients with different neurological disorders. Although 14-3-3 gamma played critical physiological or pathological role in brain, it has not been reported on brain tumorigenesis. To test expression of 14-3-3 gamma in brain tumor, 3 brain tumor cell lines and 4 normal brain tissues, 24 astrocytoma, 14 glioblastoma mutiform, 2 oligodenroglioma, 1 ependymoma were analyzed using RT-PCR, western blotting, immunohistochemistry, real-time quantitative PCR. The study found that the expressions of 14-3-3 gamma mRNA in all of tumor three cell lines was greater than normal brain tissue, but the 14-3-3 gamma proteins expressed were lower than normal brain tissue. In brain tumor tissues, higher 14-3-3 gamma mRNA expression was detected in 20 of 24 astrocytoma (83%) and higher 14-3-3 gamma protein expression was detected in 9 of 24 astrocytoma (37%). The expression of 14-3-3 gamma mRNA is higher than normal brain tissue in all 14 glioblastoma multiforme (100%), and the 14-3-3 gamma protein was expressed higher in 9 of 14 glioblastoma multiforme than normal brain tissue (64.3%). Besides, the 14-3-3 gamma protein expressed much higher in glioblastoma multiforme than astrocytoma .The copy number of the 14-3-3 gamma gene was higher in astrocytoma and glioblastoma multiforme than normal brain tissue. Thus, this study evidenced that the 14-3-3 gamma protein may play a crucial role during tumorigenesis of brain tumors.
| Identifer | oai:union.ndltd.org:NSYSU/oai:NSYSU:etd-0909104-170854 |
| Date | 09 September 2004 |
| Creators | Kao, Chiu-li |
| Contributors | Chung-lung Cho, Hung-sheng Hsia, Han-jung Chen |
| Publisher | NSYSU |
| Source Sets | NSYSU Electronic Thesis and Dissertation Archive |
| Language | Cholon |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0909104-170854 |
| Rights | not_available, Copyright information available at source archive |
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