This PhD thesis has been proposed to validate and then apply innovative analytical methodologies
for the determination of compounds with harmful impact on human health, such as biogenic amines
and ochratoxin A in wines. Therefore, the influence of production technology (pH, amino acids
precursor and use of different malolactic starters) on biogenic amines content in wines was
evaluated.
An HPLC method for simultaneous determination of amino acids and amines with precolumnderivatization
with 9-Fluorenyl-methoxycarbonyl chloride (FMOC-Cl) and UV detection was
developed.
Initially, the influence of pH, time of derivatization, gradient profile were studied. In order to
improve the separation of amino acids and amines and reduce the time of analysis, it was decided to
study the influence of different flows and the use of different columns in the chromatographic
method. Firstly, a C18 Luna column was used and later two monolithic columns Chromolith in
series. It appeared to be suitable for an easy, precise and accurate determination of a relatively large
number of amino acids and amines in wines.
This method was then applied on different wines produced in the Emilia Romagna region. The
investigation permitted to discriminate between red and white wines. Amino acids content is related
to the winemaking process. Biogenic amines content in these wines does not represent a possible
toxicological problem for human health.
The results of the study of influence of technologies and wine composition demonstrated that pH of
wines and amino acids content are the most important factors. Particularly wines with pH > 3,5
show higher concentration of biogenic amines than wines with lower pH. The enrichment of wines
by nutrients also influences the content of some biogenic amines that are higher in wines added with
amino acids precursors. In this study, amino acids and biogenic amines are not statistically affected
by strain of lactic acid bacteria inoculated as a starter for malolactic fermentation.
An evaluation of different clean-up (SPE-MycoSep; IACs and LLE) and determination methods
(HPLC and ELISA) of ochratoxin A was carried out. The results obtained proved that the SPE
clean-up are reliable at the same level while the LLE procedures shows lowest recovery. The
ELISA method gave a lower determination and a low reproducibility than HPLC method.
| Identifer | oai:union.ndltd.org:unibo.it/oai:amsdottorato.cib.unibo.it:1056 |
| Date | 23 May 2008 |
| Creators | Corzani, Claudia <1974> |
| Contributors | Arfelli, Giuseppe |
| Publisher | Alma Mater Studiorum - Università di Bologna |
| Source Sets | Università di Bologna |
| Language | English |
| Detected Language | English |
| Type | Doctoral Thesis, PeerReviewed |
| Format | application/pdf |
| Rights | info:eu-repo/semantics/openAccess |
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