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Analyzing the 16S rDNA to Monitor the Dynamic Microbial Communities in Petroleum Polluted Soil

In this study, we had established a 16S rDNA-DGGE analys is system to detect the microbial community in petroleum polluted soil and assess the feasibility of using this system to monitor the bioremediation process. Three genomic DNA extracted methods, the KIT, the Bead-beating system, and the Freeze-thaw method were used
to evaluate the DNA extraction efficiency and purity. These DNA samples were further tested by DGGE to analysis the microbial community in soil samples. The results showed that KIT method performed advantageous not only in the DNA extraction efficiency and purity, but also expressed the richest bacterial community in its
PCR products. From the DGGE analysis, our data indicated that composition of bacterial community were different in the soil samples
that were taken from the same site but at different time. This indicated that the species and number of microorganisms in a polluted soil were under a dynamic transition. The combination of DGGE and 16S rDNA gene sequence analysis system were also proven useful in identifying the predominant microbes in a soil sample and monitoring its bacterial community.

Identiferoai:union.ndltd.org:NSYSU/oai:NSYSU:etd-0710103-100048
Date10 July 2003
CreatorsChen, Hung-Yi
Contributorsnone, none, JKL
PublisherNSYSU
Source SetsNSYSU Electronic Thesis and Dissertation Archive
LanguageCholon
Detected LanguageEnglish
Typetext
Formatapplication/pdf
Sourcehttp://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0710103-100048
Rightsoff_campus_withheld, Copyright information available at source archive

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