1. Spirodela oligorrhiza on sterile glucose-mineral medium ceased to grow three days after transfer into darkness. 2. Cytokinins, supplied in the medium, allowed continuous growth of Spirodela after transfer into darkness. Other plant growth substances, or adenine analogues, were ineffective. 3. Kinetin stimulated production of new fronds after a 24 hour lag period when added to dormant cultures although it was rapidly taken up. Kinetin reached a constant concentration in the plantlets within 30 to 60 minutes of addition to the medium. 4. In the absence of cytokinin, dormancy continued for three or four weeks after which growth spontaneously resumed in darkness. The growth rate then reached almost half that achieved with optimum kinetin concentrations in darkness. Growth continued in darkness for at least eight weeks without cytokinin. 5. Pretreatment in the light with either metabolic inhibitors, kinetin, abscisic acid, or high or low temperature, essentially eliminated the period of dormancy of Spirodela transferred to darkness in the absence of cytokinin. Growth was reduced 50% to 90% during the pretreatments. 6. The pretreatments designed to affect plastid RNA, protein or ATP production were the most effective. The fastest growth rate achieved in darkness without cytokinin after pre-treatment was 10% less than that promoted by optimum kinetin. 7. Dormant Spirodela in darkness continued to incorporate precursors into RNA, DNA and protein at a rate 50% that in growing (plus kinetin) cultures. Net rates of macromolecule accumulation were extremely slow, indicating extensive degradation. 8. Addition of kinetine to non-growing Spirodela in darkness stimulated the synthesis of RNA, DNA and protein simultaneously after a lag of approximately one hour. The rates of precursor incorporation increased to equal those in continuously growing cultures. 9. Non-growing Spriodela in darkness rapidly accumulated starch. Kinetin had little or no effect on accumulation and mobilisation of starch. 10. 14C-glucose was taken up by growing (plus kinetin) and non-growing (minus kinetin) Spirodela in darkness, and was metabolized equally in each. Three times as much 14C-glucose entered starch in the non-growing cultures. 11. A model scheme for control of dormancy in Spirodela is proposed based on an inhibitory mediator. The mediator may be similar to the hypothetical mediator of the pleiotypic response shown by animal cells.
| Identifer | oai:union.ndltd.org:ADTP/278069 |
| Date | January 1971 |
| Creators | McCombs, Patrick James Alan, 1947- |
| Publisher | ResearchSpace@Auckland |
| Source Sets | Australiasian Digital Theses Program |
| Language | English |
| Detected Language | English |
| Rights | Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated., http://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm, Copyright: The author |
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