Gelatin methacrylate (GelMA) is a ubiquitous biocompatible photopolymer used in tissue engineering and regenerative medicine due to its cost-effective synthesis, tunable mechanical properties, and cellular response. Biotechnology applications utilizing GelMA have ranged from developing cell-laden hydrogel networks to cell encapsulation and additive manufacturing (3D printing). However, extrusion based 3D printing is the most common technique used with GelMA. Mask projection microstereolithography (MPµSL or µSL) is an advanced 3D printing technique that can produce geometries with high resolution, high complexity, and feature sizes unlike extrusion based printing. There are few biomaterials available for µSL applications, so 3D printing GelMA using µSL would not only add to the repertoire materials, but also demonstrate the advantages of µSL over other 3D printing techniques. A novel GelMA resin was tested with µSL to create a porous scaffold with a height and print time that has not been displayed in the literature before for a scaffold of this size. The resin consists of GelMA, deionized water, lithium phenyl-2,4,6-trimethylbenzoylphosphinate (LAP, photoinitiator), and 2-Hydroxy-4-methoxybenzophenone-5-sulfonic acid (sulisobenzone, UV blocker) and can be processed at room temperature. Four resins were tested (w/w %) and characterized for µSL printing: 20% GelMA 0.5% UV blocker, 20% GelMA 1.0% UV blocker, 30% GelMA 0.5% UV Blocker, and 30% GelMA 1.0% UV blocker. Swell testing, working curve, photo-rheology, photo-DSC (dynamic scanning calorimetry), 3D printing, and cell culture tests were performed and results showed that 30% GelMA 1.0% UV blocker had the best 3D print fidelity among resin compositions. / Master of Science / Three dimensional (3D) printing is a widely used technology to rapidly produce structures with varying degrees of complexity. 3D printing of biological components is of interest because as the world population increases, there is a lack of donors available to compensate for organ loss and tissue replacement. 3D printing offers a solution to great custom scaffolds and structures that mimic physiological geometry and properties. One printing technique is known as microstereolithography, or µSL, which uses a projector-like system to pattern ultraviolet (UV) light in specific arrangements to generate complex geometries and 3D parts. Gelatin is a material of interest for this technology because gelatin is derived from collagen, which is the most abundant protein found in the body. Gelatin can be modified so that it is reactive with UV light, and can be processed with µSL to generate 3D structures. In this work, gelatin was modified into the form of gelatin methacrylate (GelMA) in order to develop and test resin formulations for use with µSL. Four different resins were tested and characterized and the results indicated that one GelMA resin produced prints with greater fidelity and resolution than other formulations. This resin has been identified for potential applications in tissue engineering and 3D printed organ development.
| Identifer | oai:union.ndltd.org:VTETD/oai:vtechworks.lib.vt.edu:10919/101053 |
| Date | 18 June 2019 |
| Creators | Surbey, Wyatt R. |
| Contributors | Materials Science and Engineering, Whittington, Abby R., Foster, Earl Johan, Yu, Hang |
| Publisher | Virginia Tech |
| Source Sets | Virginia Tech Theses and Dissertation |
| Detected Language | English |
| Type | Thesis |
| Format | ETD, application/pdf, application/pdf |
| Rights | In Copyright, http://rightsstatements.org/vocab/InC/1.0/ |
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