Consider a sequence of equally spaced points along a helix in three-dimensional space, which are observed subject to statistical noise. In this thesis, maximum likelihood (ML) method is developed to estimate the parameters of the helix. Statistical properties of the estimator are studied and comparisons are made to other estimators found in the literature. Methods are established here for the fitting of unkinked and kinked helices. For an unkinked helix an initial estimate of a helix axis is estimated by a modified eigen-decomposition or a method from the literature. Mardia-Holmes model can be used to estimate the initial helix axis but it is often not very successful one since it requires initial parameters. A better method for initial axis estimation is the Rotfit method. If the the axis is known, we minimize the residual sum of squares (RSS) to estimate the helix parameters and then optimize the axis estimate. For a kinked helix, we specify a test statistic by simulating the null distribution of unkinked helices. If the kink position is known, then the test statistic approximately follows an F-distribution. If the null hypothesis is rejected i.e. the helix has a change point, and then cut the helix into two sub-helices between the change point where the helix has the maximum statistic. Statistics test are studied to test how differ these two sub-helices from each other. Parametric bootstrap procedure is used to study these statistics. The shapes of protein alpha-helices are used to illustrate the procedure.
| Identifer | oai:union.ndltd.org:bl.uk/oai:ethos.bl.uk:755152 |
| Date | January 2018 |
| Creators | Alfahad, Mai F. A. M. |
| Contributors | Kent, John T. ; Mardia, Kanti V. |
| Publisher | University of Leeds |
| Source Sets | Ethos UK |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Source | http://etheses.whiterose.ac.uk/21675/ |
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