Measurement of enhancements to the nuclear magnetic spin-lattice relaxation times of solvent water protons were used to probe the complexation of manganese(II) ions with the iron protein of nitrogenase. Enhancement data were used to determine concentrations of bound manganese in solutions containing the metal ions and buffered aqueous protein, and these concentration data treated by the Scatchard plot method to provide binding parameters for the manganese-protein complex. These studies showed four independent, equivalent sites on the protein for binding manganese ions. Competition studies in solutions containing the iron protein and known concentrations of manganese(II) and magnesium(II) ions showed that the four sites available to manganese could alternatively bind magnesium ions.
| Identifer | oai:union.ndltd.org:bl.uk/oai:ethos.bl.uk:467721 |
| Date | January 1978 |
| Creators | Orchard, David |
| Publisher | University of Sussex |
| Source Sets | Ethos UK |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
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