This thesis contains work primarily pertaining to two different projects, with the majority of work carried out at Queen's University of Belfast, QUS, along with some at TUDelft, Delft University of Technology, Delft, NL. The first project was concerned with the development, optimisation, and implementation of high throughput inexpensive, and facile toxicity testing methods for use with novel ionic liquids. Approximately one hundred ionic liquids were tested against two strains of bacteria - one Gram negative and one Gram positive. The results varied from no-to-low inhibit ion zones to large inhibition zones, indicating toxicity to the organisms tested. The second project was concerned with the use of ionic liquids as co-solvents in a biocatalytic reaction involving novel enoate reductase enzymes. Approximately twenty ionic liquids, both water-miscible and water-immiscible were tested. Two methods were used for the analysis of the reactions performed, ultraviolet spectrophotometric analysis and liquid -liquid extraction combined with gas chromatographic analysis. The water-immiscible ionic liquids garnered better results when tested with the enzymes of interest than did the water-miscible ones.
| Identifer | oai:union.ndltd.org:bl.uk/oai:ethos.bl.uk:603419 |
| Date | January 2013 |
| Creators | Tether, Angela Lynn |
| Publisher | Queen's University Belfast |
| Source Sets | Ethos UK |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
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