The intention throughout this work has been to correlate the many factors involved in the metabolism of the nitrogen fixing species A. vinelandii in order to present a comprehensive unified whole. The various techniques employed have been strictly adhered to, after a probationary trial period used to evaluate their different potentialities; with the result that comparison of values obtained over a period of some months has been attempted. Continuous culture has been shown to be a useful asset in the production of cell material and to have possibilities of an interesting development in further studies of the growth metabolism of the bacteria. The ultrasonic method of disruption of Azotobacter has been extended and developed into a satisfactory routine procedure associated with centrifugal separation. An investigation of the third step in the reduction of nitrogen (i.e. N - N bond reduction) has been carried out and forms a stage in the complete examination of reduction of nitrogen-nitrogen compounds undertaken in this laboratory. Electron microscopy has been used to examine the progress of disruption and also to establish some morphological details of the bacteria.
| Identifer | oai:union.ndltd.org:bl.uk/oai:ethos.bl.uk:602129 |
| Date | January 1962 |
| Creators | Stewart, Michael Charles |
| Publisher | Imperial College London |
| Source Sets | Ethos UK |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Source | http://hdl.handle.net/10044/1/13564 |
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