A Series of ruthenium(II)-(tpm)(dppz), (phen) and (dppn) complexes have been synthesised which have been designed to show binding to DNA. These complexes have been characterized by a wide range of techniques, including X-ray crystallography. The binding of the complexes with CT-DNA sequence has been studied using a variety of techniques, including, UV-Vis, luminescence and CD spectroscopy as well as viscometry. hi most of the cases, the metal complexes stabilise DNA, bind via intercalation and their binding affinities with CT-DNA are in the micromolar range. We have observed a remarkable modulation of the binding of our complexes to DNA by changing the ancillary ligands of the complex. As a comparison DNA binding properties of some Ru(II)-(tpy)(dppz) and Ru(II)-(tpy)(phen) complexes synthesised in the same way were also explored. These metal complexes stabilise DNA and viscosity measurements also shows intercalation between base pairs.
| Identifer | oai:union.ndltd.org:bl.uk/oai:ethos.bl.uk:554927 |
| Date | January 2011 |
| Creators | Mlitan, Adel M. |
| Publisher | University of Sheffield |
| Source Sets | Ethos UK |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
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