This thesis describes the work undertaken in order to improve the resolution of protein mixtures using ion exchange chromatography on cellulosic ion exchange materials. The thesis consists of two parts. The first part describes the modification of existing cellulosic material, in order to improve the physicochemical and chromatographic properties of the ion exchanger. This is done through the introduction to DCAY cellulose, an anion exchange material, of crosslinking agents of different length and also by the introduction to cellulose of a previously untried ion exchange group. The second part of the thesis describes a method of employing stepped Had elution gradients to give greatly improved protein resolution in bovine serum and human plasma samples on small and large columns of DIME cellulose. A method of pN/ionic strength gradient elution on CM cellulose is described using acetate and phosphate buffers for bovine aatum and human plasma chromatography and ionic strength gradient and pit gradient solution methods are described for the chromatography of milk whey proteins. A method of NAM gradient elution on CH cellulose is also described, which, when used in conjunction with the stepped elution gradient method greatly inproves the protein resolution of bovine serum, human plasma and milk whey samples. This method of elution is also applied to blood plasma samples from a person suffering from a blood disorder known an macroglobulinemia.
| Identifer | oai:union.ndltd.org:bl.uk/oai:ethos.bl.uk:636773 |
| Date | January 1978 |
| Creators | Edwards, W. G. |
| Publisher | Swansea University |
| Source Sets | Ethos UK |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
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