This thesis describes the development of the various methods available for the synthesis of deoxyoligoribonucleotides. The use of the 2,2-bis (4-nitro-phenyl)ethyl group was investigated, mainly as a 5'-hydroxyl protecting group on 2'-deoxyribonucleotides, but also as a base protecting group for the O4-function of thymidinc and the Nc-exocyclic amine of adenosine. The deprotection conditions required for the removal of this group and its subsequent use in the synthesis of 2'-deoxyribonucleotides utilising phosphoramidite methodology arc described. An investigation, by 81P n.m.r., of the coupling and oxidation steps of the synthesis of DNA is included as well as a brief study of the potential use of zinc iodide as an accelerating agent in the coupling of nuclcotidcs by the phosphotriester method of synthesis.
| Identifer | oai:union.ndltd.org:bl.uk/oai:ethos.bl.uk:663106 |
| Date | January 1990 |
| Creators | Turner, Gillian |
| Publisher | University of Edinburgh |
| Source Sets | Ethos UK |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Source | http://hdl.handle.net/1842/13149 |
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