Methodology for the synthesis of peptide <i>C</i>-terminal aldehydes has been investigated. Modification of a linker system, based on a terabenzosuberyl construct, has been demonstrated to be suitable for the synthesis of peptide <i>C</i>-terminal semicarbazones. The route has been fully optimised to yield a series of peptide <i>C</i>-terminal semicarbazones and the corresponding peptide aldehydes. The chemical synthesis of deglycosylated human interferon-gamma (143 residues) has been carried out. The purification of this protein has been investigated and a short purification protocol developed which is sufficiently general to allow application to other similar protein systems. Purification and characterisation of the synthetic interferon-gamma molecule has been completed and folding of the molecule attempted.
| Identifer | oai:union.ndltd.org:bl.uk/oai:ethos.bl.uk:660438 |
| Date | January 1999 |
| Creators | Patterson, Jennifer Ann |
| Publisher | University of Edinburgh |
| Source Sets | Ethos UK |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Source | http://hdl.handle.net/1842/12777 |
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