Mechanical cues play important roles in controlling cell function and have implications fo1' generating functional tissues for clinical use. This thesis investigates the potential of using magnetic particle (MNP) mediated mechanical stimulation techniques through Magnetic Force Bioreactor (MFB) to alternatively activate mechanical sensitive cell membrane receptors for the tissue engineering applications and the study of mechanotransduction. In this study, broad cell types including primary human mesenchymal stem cells (hMSCs), transformed mouse embryonic stem cells (mESCs) and HEK293 cells have been used and two different strategies for improving selective targeting cell membrane receptors, in that antibodies or peptides are employed as functionalizing biomolecules to facilitate various cell membrane receptors targeting, have been utilized. The long term application of mechanical stimulation in the osteogenic induction of hMSCs and mESCs mediated by MNPs has been undertaken. The cellular response to MNP mediated stimulation has becn examined by immunofluorescence, ELISA, qRT-PCR, histology, FTIR and uCT.
| Identifer | oai:union.ndltd.org:bl.uk/oai:ethos.bl.uk:602978 |
| Date | January 2013 |
| Creators | Hu, Bin |
| Publisher | Keele University |
| Source Sets | Ethos UK |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
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