The spindle assembly checkpoint (SAC) functions to prevent anaphase onset until all chromosomes are correctly bi-oriented on the mitotic spindle and aligned at the metaphase plate. Cohesion between sister chromatids is essential for this biorientation. In animal cells, most cohesin is removed from chromosome arms during prophase and prometaphase. Cohesin at centromeres is refractory to removal at this stage and persists until metaphase, whereupon its Sccl subunit is cleaved by separase, which is thought to trigger anaphase. What protects centromeric cohesin from the prophase pathway? 1 show that depletion of Sgol by RNA interference in HeLa cells causes precocious loss of centromeric cohesin from chromosomes in prometaphase and a permanent cell cycle arrest, presumably due to inactivation or the spindle checkpoint.
| Identifer | oai:union.ndltd.org:bl.uk/oai:ethos.bl.uk:490111 |
| Date | January 2008 |
| Creators | McGuinness, Barry E. |
| Publisher | University of Oxford |
| Source Sets | Ethos UK |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
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