The three different platform candidates were antibody microarray, surface Plasmon resonance (SPR) and bead array. Another objective of this project was to explore other applications of these platforms, and the selected example application was to explore other applications of these platforms for hybridoma screening. Since antibodies against plant pathogens have previously been produced, characterized and used in development of the three different platforms, to examine the possibility of applying the platforms to facilitate the screening of hybrid om a, pilhogenic foodbome bacterium Listeria monocytogenes was used as a model study. In summary, antibody array and bead array technologies were demonstrated to be able to detect multiple plant pathogens at the same time. Both were shown to be able to detect the pathogens in the real samples from fields. making them good candidates for plant industries. On the other hand, despite considerable effort to incr¢ase the sensiritivity of SPR, lower limit of detection could not be achieved. This courd be an intrinsic limitation of this method for detecting a whole cell where the size of the analytic is bigger than usual SPR analytes such as small compounds. From these technologies. an antibody array was selected for hybridoma screening and was shown to be effective, albeit with lower sensitivity compared to ELISA. The antibody array proved to be a faster and simpler method to screen hundreds of hybricioma 3 clones. A comnbination of biosensor tcchnology ensures good quality screening for hybridoma clones as demonstrated in this study.
| Identifer | oai:union.ndltd.org:bl.uk/oai:ethos.bl.uk:600640 |
| Date | January 2013 |
| Creators | Charlermroj, Ratthaphol |
| Publisher | Queen's University Belfast |
| Source Sets | Ethos UK |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
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