The human AIFM2 protein (formerly known as AMID) is an apoptosis associated flavoprotein with a 6-hydroxy FAD cofactor. AIFM2 is a NAD(P)H-binding oxidoreductase with some sequence similarities to A1FM1 (formerly known as AIF, Apoptosis Inducing Factor), a mitochondrion-associated enzyme which relocates to the cell nucleus during apoptosis and Is considered to be a key player in the progression of cell death (1). Uniquely, AIFM2 binds 100% 6-hydroxy FAD as a cofactor. The complete 6-hydroxylation of the FAD cofactor in AIFM2 is auto-catalysed only in the presence of NADPH, and AIFM2 is purified from an E Coli expression system as the completely 6-hydroxylated form, which Is green in colour (2).
| Identifer | oai:union.ndltd.org:bl.uk/oai:ethos.bl.uk:502385 |
| Date | January 2009 |
| Creators | Collins, Hannah F. |
| Publisher | University of Manchester |
| Source Sets | Ethos UK |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
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