Enhanced ethanol fennentation is one of the current issues in fennentation technology; this also is a main aim that goes through this thesis. In this thesis, S. cerevisiae KAY446 was used for ethanol fennentation under very high gravity (VHG) conditions. This strain showed an ability to fennent under these conditions since an average ethanol concentration of 117.5 giL was detennined for continuous fennentation. In tenns of deeper biological aspects, a proteomic analysis of S. cerevisiae was also perfonned to gain an understanding of this micro-organism in responding to stress conditions due to high glucose concentrations in the broths. Therefore, the iTRAQ teclmique was used as a proteomics tool for this purpose. The reliability of this technique was analysed via three different domains of life, including S. cerevisiae (eukaryotes), S. solfatarictls (archaea) and SynecllOcystis sp. (bacteria). Based on multiple replicate analyses, an increment of peptidcs (proteins) was found, as well 'as a tcchnical replicate to confinn the reliability of this method. The main functions of the protcomic analyses in this thesis were to discover three crucial objectives: firstly, to Investigate the cellular response of S. cerevisiae to osmotic stress generated by VHG conditions, as well as to immobilization of cells; secondly, to examine the response of this micro-organism to VHG conditions with an amino acids supplementation; and finally, to investigate the main triggers for the fluctuation of fennentation parameters observed during continuous fennentation process.
| Identifer | oai:union.ndltd.org:bl.uk/oai:ethos.bl.uk:489864 |
| Date | January 2008 |
| Creators | Khoa, Trong |
| Publisher | University of Sheffield |
| Source Sets | Ethos UK |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
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