The nucleic acids, RNA in particular, are conformationally complex biomolecules. The structures that they can form and the subtle sequence dependent modifications of conformation are of pivotal importance to their function <I>in vivo</I>. The advent of solid phase chemical synthesis methodologies for DNA and, more recently RNA, has fuelled the rapid growth in our knowledge of nucleic acid structure. The work described here is concerned with the development of rapid and reliable synthesis and purification protocols for the automated chemical synthesis of RNA. The use of RNA phosphoramidite monomers incorporating two different 2'-hydroxyl protecting groups is described, along with ion-exchange and reversed phase HPLC protocols for the purification of oligoribonucleotides. The quality of the material obtained by these methods has allowed detailed biophysical investigations by UV melting, high field NMR and X-ray crystallography.
| Identifer | oai:union.ndltd.org:bl.uk/oai:ethos.bl.uk:643379 |
| Date | January 1996 |
| Creators | Conn, Graeme Leslie |
| Publisher | University of Edinburgh |
| Source Sets | Ethos UK |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Source | http://hdl.handle.net/1842/12169 |
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