The versatility of bacteriophage lambda as a vector for the amplification of DNA fragments has been limited by the technical difficulties involved in preparing vectors possessing one or two targets for particular restriction enzymes. In this thesis, the use of 'adapter fragments', i.e., short, naturally occurring DNA fragments terminated by the recognition sequence for a different restriction enzyme at each end, has been studied. Such fragments have been employed to insert a DNA fragment produced by R.T digestion into a vector R.Hin III target. The need to isolate adapter fragments has been obviated by using genetic or physical screening or selection for the insertion of adapter fragments. Two DNA fragments of interest from plasmid recombinants have been transferred to lambda recombinants using these techniques.
| Identifer | oai:union.ndltd.org:bl.uk/oai:ethos.bl.uk:662881 |
| Date | January 1979 |
| Creators | Thompson, Richard |
| Publisher | University of Edinburgh |
| Source Sets | Ethos UK |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Source | http://hdl.handle.net/1842/13112 |
Page generated in 0.0017 seconds