The effects on coliphage T6r of 11 commonly used chemical antibacterial agents have been examined. The effect of the agents on the multiplication of the phage was shown by their effect on the mass lysis of fluid cultures of the host by the phage. Only aminacrine hydrochloride and sodium lauryl sulphate showed significant inhibition of the growth of the phage at concentrations below those inhibiting the host. For the examination of the effect of the agents on free phage particles, a method of assessment giving precise inactivation time estimates has been applied and two methods of interpreting the results have been compared. Large differences have been shown in the effectiveness of different antibacterial agents in inactivating the free phage. The effect on the inactivation time of changes in concentration of the antibacterial agent has been examined for five of the agents and the regression between log inactivation time and log concentration has been shown to be linear. The dilution coefficients for these agents fell into two distinct groups, those for chloramine T and formaldehyde (about 2 and 3 respectively) being very much smaller than those for crystal violet, cetrimide and phenol (about 11,13 and 15 respectively). It has been postulated that this difference in dilution coefficients indicates different mechanisms of inactivation between the two groups and the possible nature of the mechanisms has been discussed. Methods of cultivation of the phage and its stability under different conditions of storage have been investigated. A method of plaque counting by surface drop plating has been developed and its reproducibility examined.
| Identifer | oai:union.ndltd.org:bl.uk/oai:ethos.bl.uk:602152 |
| Date | January 1962 |
| Creators | Brown, William Robert Laing |
| Contributors | Cook, A. M. |
| Publisher | Imperial College London |
| Source Sets | Ethos UK |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Source | http://hdl.handle.net/10044/1/13639 |
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