The chromosome of a slowly-growing E. coli cell has a previously unappreciated level of spatial organization. By analyzing the localization patterns of multiple segregated genetic loci and locus pairs in fixed cells, I demonstrated that the left (L) and the right (R) replichores of the circular chromosome locate to opposite halves of each daughter chromosome with 1 replication origin (oriC) in the middle. Furthermore, loci on each replichore locate in a linearmannerfromonCto one of the chromosome edge, roughly recapitulating their genetic positions on the genome map.
| Identifer | oai:union.ndltd.org:bl.uk/oai:ethos.bl.uk:490106 |
| Date | January 2008 |
| Creators | Lui, Xun |
| Publisher | University of Oxford |
| Source Sets | Ethos UK |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
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