Listeria monocytogenes is a Gram-positive, facultative intracellular pathogen which causes listeriosis, an important food-borne infection, with a high fatality rate. Temperature and growth media constituents have been previously shown to repress virulence factor expression in L monocytogenes but the mechanisms of control surrounding this virulence factor repression are poorly understood. Using both functional and biochemical assays to measure virulence factors, particularly llsteriolysin, and molecular techniques such as real-time PCR and Northern blot analysis to analyse the transcription or Key virulence genes, particularly hlyA, in the presence or absence of carbohydrates it was hoped a better understanding of virulence factor control could elucidated.
| Identifer | oai:union.ndltd.org:bl.uk/oai:ethos.bl.uk:496153 |
| Date | January 2008 |
| Creators | McCulloch, E. J. |
| Publisher | Glasgow Caledonian University |
| Source Sets | Ethos UK |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
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