Aneuploidy is defined as a state of having an abnormal number of chromosomes within a cell. In human, congenital aneuploidy is the leading cause of developmental abnormality and mental retardation. Somatically acquired aneuploidy has long been known as a hallmark of cancer genome. While multiple studies have focused on dissecting the molecular mechanisms leading to aneuploidy, little has been done to address its consequences. Using budding yeast Saccharomyces cerevisiae as model organism, we first asked whether and how aneuploidy could bring about phenotypic variation. We monitored the growth of a panel of 38 isogenic and relative stable aneuploid strains under various conditions including a panel of chemotherapeutic and antifungal drugs. We found that some aneuploid strains grew significantly faster than euploid control strains in various conditions. Using quantitative proteomic, we showed that aneuploidy could induce large change to the cellular proteome and the levels of protein expression largely scale with chromosome copy numbers. Together, these findings suggest that aneuploidy, through its effect on proteome, could underlie phenotypic variation.
| Identifer | oai:union.ndltd.org:bl.uk/oai:ethos.bl.uk:607484 |
| Date | January 2013 |
| Creators | Zhu, Jin |
| Publisher | Open University |
| Source Sets | Ethos UK |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
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